• Product NameMBP Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• PurificationThe antibody was purified by immunogen affinity chromatography.
• ApplicationsWB,IHC
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total MBP protein.
• Immunogen TypePeptide
• Immunogen DescriptionKLH-conjugated synthetic peptide encompassing a sequence of human MBP. The exact sequence is proprietary.
• Target NameMBP
• Alternative Names    MGC99675;
• Accession No. Swiss-Prot:P02686
  NCBI Gene ID:4155
• UniprotP02686
• Gene ID4155;
• Calculated MW Predicted band size: 33 kDa
  
• SDS-PAGE MW Observed band size: 18;24 kDa
• Concentration 1.0mg/ml
• Formulation Liquid in PBS, pH 7.3, 50% glycerol, and 0.05% Proclin300.
• Storage Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• WB 1:500 - 1:1000;
  IHC 1:50 - 1:200;
  

MBP belongs to the myelin basic protein family. The classic group of MBP isoforms (isoform 4-isoform 14) are the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. This is a rabbit polyclonal antibody raised against the full-length of human MBP,and is capable of recognizing multiple isoforms of MBP.

• Western blot analysis of MBP expression in mouse brain (A), rat brain (B) whole cell lysates.
  (Predicted band size: 33 kDa; Observed band size: 18; 24 kDa)
• Immunohistochemical analysis of MBP staining in human brain formalin fixed paraffin embedded tissue section.
  The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0).
  The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system.
  DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting